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Sarstedt t25 flasks
T25 Flasks, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t25+flasks/flasks+t75/10__1096_slash_fj__202600816rr-34-9-11
Average 86 stars, based on 1 article reviews
t25 flasks - by Bioz Stars, 2026-09
86/100 stars

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Cell Culture:

Article Title: Image quality metrics fail to accurately represent biological information in fluorescence microscopy
Article Snippet: .. Cells were cultured in T25 flasks (Sarstedt) with fibroblast culture media (high glucose DMEM (Gibco) supplemented with 10% FBS (Hyclone), 1% sodium pyruvate (Gibco), 1% penicillin-streptomycin (Sigma) and 4 mM L-Glutamine (Gibco)) and maintained at 37 °C and 5% CO2 until reaching approximately 80-90% confluency. ..

Article Title: Cytoprotective and Genoprotective Effects of Gelatin-Encapsulated Quercetin Against Oxidative Cell Damage
Article Snippet: .. Cells were cultured in T25 flasks (Sarstedt, Newton, NC, USA) in DMEM supplemented with 10% FBS under standard conditions at 37 °C in a humidified atmosphere containing 5% CO 2 . .. For experiments, cells were seeded into 96- and 24-well plates (Sarstedt, USA).

Article Title: MicroRNA-132 regulates quinolinic acid production in the brain during LPS-induced neuroinflammation
Article Snippet: Murine BV-2 microglial cells were purchased from Cell Lines Service (Cytion, catalog number 305156). .. Upon receipt, BV-2 microglia were cultured in T25 flasks (Sarstedt) containing Roswell Park Memorial Institute (RPMI 1640) medium (Wisent Inc, Canada) supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin (SH40003.01, Cytiva, Austria) at 37°C in a humidified atmosphere containing 5% CO2. ..

Article Title: Passage‐Dependent Size Distributions of Human‐Derived Cells: Implications for Metabolic Assays
Article Snippet: .. Before each round of experiments, cells were cultured in T25 flasks (Sarstedt, Numbrecht, Germany) in an incubator (37°C, 5% CO2, 95% RH) according to the cell providers' guidelines (see Table 1). ..

Incubation:

Article Title: Antarctic fish cell cultures show adaptation of organelle morphology and dynamics to extreme cold
Article Snippet: Media were then resterilised using a 0.2 μm membrane filter (Fisher Scientific, 15206869). .. COS-7 cells were grown in T75 flasks (Greiner, 658175) or T25 flasks (Sarstedt, 83.3910.002) by incubation at 37 °C in a 5% CO 2 atmosphere under controlled humidity. .. Complete medium for normal cell growth consisted of 90% high-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, D6546), 10% FBS (Gibco, A5256801), 1% GlutaMAX (Gibco, 35050), and 1% Antibiotic-Antimycotic (Gibco, 15240).

Article Title: New Glass-Ceramics in the System Ca 2 SiO 4 -Ca 3 (PO 4 ) 2 —Phase Composition, Microstructure, and Effect on the Cell Viability
Article Snippet: After thawing, HL-60 cells were washed by centrifuging at 200× g with pre-warmed 10% FBS/RPMI medium supplemented with amino acids (#R7131, Sigma-Aldrich, Darmstadt, Germany) and antibiotics (#R8758, Sigma-Aldrich, Germany). .. The cells were seeded at concentration 1 × 10 6 mL −1 in T25 flasks (#83.3910.002, Sarstedt, Germany) and incubated at 37 °C, 5% CO 2 (Memmert incubator, Schwabach, Germany). .. After 48 h exponential growth, the cells were collected and treated with vitamin D3 (VitD3) (#5009360010, Merck, Darmsrtadt, Germany) at a concentration of 10 −10 M to obtain osteoclast-like morphology as described by Yoneda et al., 1991 [ ].

Concentration Assay:

Article Title: New Glass-Ceramics in the System Ca 2 SiO 4 -Ca 3 (PO 4 ) 2 —Phase Composition, Microstructure, and Effect on the Cell Viability
Article Snippet: After thawing, HL-60 cells were washed by centrifuging at 200× g with pre-warmed 10% FBS/RPMI medium supplemented with amino acids (#R7131, Sigma-Aldrich, Darmstadt, Germany) and antibiotics (#R8758, Sigma-Aldrich, Germany). .. The cells were seeded at concentration 1 × 10 6 mL −1 in T25 flasks (#83.3910.002, Sarstedt, Germany) and incubated at 37 °C, 5% CO 2 (Memmert incubator, Schwabach, Germany). .. After 48 h exponential growth, the cells were collected and treated with vitamin D3 (VitD3) (#5009360010, Merck, Darmsrtadt, Germany) at a concentration of 10 −10 M to obtain osteoclast-like morphology as described by Yoneda et al., 1991 [ ].



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